...in human disease: lymphokine patterns in leprosy. Chem...

来自 : www.oalib.com/references/4662. 发布时间：2021-03-24

ABSTRACT: Background Patients with localized tuberculoid and generalized lepromatous leprosy show respectively Th1 and Th2 cytokine profile. Additionally, other patients in both types of leprosy also show a non discriminating Th0 cytokine profile with both interferon-γ and IL-4. The present study investigated the role of Th17 cells which appear to be a distinct subtype of Th subtypes in 19 tuberculoid and 18 lepromatous leprosy patients. Five healthy subjects with long term exposure to infection and 4 skin biopsies from healthy subjects undergoing cosmetic surgery were used as controls. Methodology/Principle Findings An array of Th17 related primers for cytokines, chemokines and transcription factors was used in real time reverse transcribed PCR to evaluate gene expression, ELISA for cytokine secretion in the supernatants of antigen stimulated PBMC cultures and flow cytometry for establishing the phenotype of the IL-17, IL-21 producing cells. Conclusions/Significance IL-17 isoforms showed significantly higher expression and release in supernatants of antigen stimulated PBMC cultures and dermal lesions of healthy contacts and tuberculoid leprosy as compared to lepromatous leprosy (p
Salgame P, Yamamura M, Bloom BR, Modlin RL (1992) Evidence for functional subsets of CD4+ and CD8+ T cells in human disease: lymphokine patterns ...Salgame P, Yamamura M, Bloom BR, Modlin RL (1992) Evidence for functional subsets of CD4+ and CD8+ T cells in human disease: lymphokine patterns in leprosy. Chem Immunol 54: 44–59. doi: 10.1159/000319113Salgame P, Yamamura M, Bloom BR, Modlin RL (1992) Evidence for functional subsets of CD4+ and CD8+ T cells in human disease: lymphokine patterns in leprosy. Chem Immunol 54: 44–59. doi: 10.1159/000319113Salgame P, Yamamura M, Bloom BR, Modlin RL (1992) Evidence for functional subsets of CD4+ and CD8+ T cells in human disease: lymphokine patterns in leprosy. Chem Immunol 54: 44–59. doi: 10.1159/000319113